Volta Labs launches automated library preparation solution for the Oxford Nanopore Ligation Sequencing Kit V14

Volta Labs, maker of the Callisto™ Sample Prep System, has announced the availability of its DNA Library Preparation Application for the Oxford Nanopore Technologies Ligation Sequencing Kit V14 (SQK-LSK114). The new Callisto™ Sample Preparation protocol, developed under Oxford Nanopore's Compatible Products Programme, enables laboratories to perform fully automated, ligation-based library preparation optimized for the PromethION platform.

In benchmarking studies and an independent clinical evaluation at University Medical Center Utrecht (UMC Utrecht), Callisto-prepared libraries achieved up to 120 gigabases (Gb) of output on a single PromethION flow cell, while delivering diagnostic-grade variant accuracy with 99.66% single nucleotide variant (SNV) recall.

The evaluation from UMC Utrecht, which was presented at AGBT 2026, confirmed that Callisto's integrated high molecular weight DNA extraction and ligation-based library preparation can support routine whole genome sequencing within workflows aligned to ISO 15189 standards.

Bert van der Zwaag from the Department of Genetics at UMC Utrecht stated, "As we evaluate whole genome sequencing for routine clinical diagnostics, having a robust, automated workflow is essential. Callisto's HMW DNA extraction and library preparation have given us the consistency and throughput we need to move toward ISO 15189 accreditation for Oxford Nanopore-based sequencing, while delivering variant calling accuracy comparable to our established short-read pipelines."

Thomas Bray, Vice President of Corporate Business Development at Oxford Nanopore Technologies, remarked, "The results achieved with libraries prepared with the Callisto™ Sample Prep System demonstrate the performance our platform can deliver when paired with high-quality sample preparation for whole genome sequencing applications. We are excited to welcome Volta Labs into our Compatible Products programme."

Founded in 2018 at MIT, Volta Labs aims to transform genomic sample preparation with the Callisto™ Sample Prep System. Utilizing proprietary electrowetting technology, Callisto offers fully automated DNA and RNA extraction and library preparation for all major sequencing platforms. The platform reduces hands-on time by up to 80% and delivers robust performance across multiple sample types and chemistries. Volta Labs is headquartered in Boston, MA.